Validation of Reference Genes for mRNA Quantification in Adjuvant Arthritis
نویسندگان
چکیده
منابع مشابه
Validation of Reference Genes for mRNA Quantification in Adjuvant Arthritis
Real time quantitative PCR (RT-qPCR) requires a method to normalize the expression of target genes against an endogenous reference gene. It is known that commonly used housekeeping genes (HKGs) vary tremendously in inflammatory conditions; however information about the stability and expression of HKGs in chronic inflammatory joint disease such as rheumatoid arthritis (RA) is scarce. The express...
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BACKGROUND Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available on suitable reference genes to be used for studies involving human epicardial adipose tissue. The objective of the curre...
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Reverse transcription of extracted cellular RNA combined with real-time PCR is now an established method for sensitive detection and quantification of specific mRNA level changes in experimental models of neurological diseases. To neutralize the impact of experimental error and make quantification more precise, normalization of test gene data using data from a constantly expressed gene, a refer...
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Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...
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ژورنال
عنوان ژورنال: Open Journal of Rheumatology and Autoimmune Diseases
سال: 2012
ISSN: 2163-9914,2164-005X
DOI: 10.4236/ojra.2012.23013